Analysis of active components and molecular mechanism of action of Rubia cordifolia L. in the treatment of nasopharyngeal carcinoma based on network pharmacology and experimental verification

The aim of this study is to explore the active components and potential molecular mechanism action Rubia cordifolia L. against nasopharyngeal carcinoma (NPC). We used network pharmacology, docking, bioinformatics analysis identify their role NPC. experimental verification was detected by MTT, AnnexinV-FITC/PI double fluorescence staining Western blotting method. Network pharmacology identified that mollugin one most effective inRubia Important NPC targets included HSP90AA1, CDK1, EGFR, PIK3CA, MAPK14, CDK2. Molecular docking revealed considerable binding activity with either 6 important targets. Bioinformatics showed these were mutated in NPC, expression CDK2 cancer tissues significantly different from normal tissues. MTT detection inhibited proliferation induced apoptosis cells. indicated related regulation Survivin XIAP. This predicted partially verified pharmacological Mollugin as a ingredient These results prove reliability approaches provide basis for further research application